Peptide Synthesis

Millipore provides custom peptide synthesis services to meet all of your research needs. A number of labels and peptide modifications are available as are unnatural amino acids, which can be incorporated at any position and in any combination. Custom peptides are synthesized by the FMOC solid phase method. All peptides are supplied lyophilized with a complete quality control package including HPLC chromatograms to ensure purity and Mass Spectral analysis to confirm identity.

Peptide Production Scale

• 5mg
• 15mg
• 25mg
• 10mg
• 10mg
• 30+ mg

Purity Grades

• Crude
• Immunograde (>70%)
• Pure Grade (>90%)
• Ultra Pure (>95%) 10mg

Modifications Available

• N-terminal
• Biotin
• Palmitylation25mg
• C-terminal FITC
• N-terminal Acetylation
• 30+ mgC-terminal Amidation
• D-form (or other unnatural)
• amino acids
• Cyclic
• MA

Cloning & Gene Expression

The molecular and cell biology group at Millipore has the experience and know-how to clone, sequence, and express your gene of interest in either prokaryotic or eukaryotic systems. Our patent portfolio includes the well known Glutathione S-Transferase (GST) fusion protein expression/purification system. This technology allows for the rapid and efficient method of expressing and purifying high quality proteins.

We offer the following services:

Peptide Production Scale

• Gene isolation and cloning
• Sequence confirmation
• Construct development

• Expression optimization
• PAGE Analysis

• High purity through affinity, ion exchange, and gel filtration chromatography
• Endotoxin removal

• DNA cloning and preparation
• Sequence confirmation for accuracy and orientation
• Transfection
• Selection and cloning (All cell lines are clonal)
• Analysis of cell lines for protein expression and functionality (low, medium, & high expressing cell lines can be generated)
• Cell line cryopreservation